Infectious disease research and vaccine drug development

● Pathogen detection: Pathogen metagenome sequencing ●HLA typing: WES, WGS ● Protein marker testing: Olink Proteomic Products ● Immune mechanism research: single cell/population RNA sequencing ● Antigen screening: single cell/population immunome testing, WES, RNA sequencing

Technological advantage

Comprehensive and in-depth research platform on immune mechanisms

From gene to protein

It not only provides a variety of transcriptome solutions such as mRNA, totalRNA, and miRNA to meet the research on immune cell expression profiles in peripheral blood or various tissues and organs, but also has a unique Olink high-throughput proteomic platform that comprehensively covers inflammation and immune response. For related proteins, up to 3000 protein markers can be detected in just a few microliters of samples, and its sensitivity to cytokines such as IL-8 reaches the Fico level.

from macro to micro

In the research of expression profiling and immune bank, we have gone from various types of RNA and human and mouse TCR/BCR detection solutions at the macro level to the research of single cells at the micro level. We have internationally recognized 10x Genomics and the domestic leading new grid element dual platform, as well as experience in processing various types of samples from humans and mice, and have rich experience in data analysis in immune cell subgroup analysis, marker expression, and clonotype research.

Case

Application of multi-omics platform to vaccine research

Stanford University researchers published an in-depth vaccinology study on Pfizer's mRNA vaccine BNT162b2 in Nature. The researchers used multiple genomic methods, including mass spectrometry flow, RNA sequencing, single-cell RNA sequencing, and plasma proteomics Olink Inflammation Panel. The study found that compared with the initial vaccination, booster vaccination significantly enhanced the innate immune response, manifested by an increase in CD14 + CD16 + inflammatory monocytes, a significant increase in plasma IFNγ concentrations and the appearance of transcriptome characteristics of congenital antiviral immunity. The researchers also used single-cell transcriptomic analysis to find that after secondary immunization, the bone marrow cell population enriched with interferon response and reduced AP-1 transcription factors increased by about 100 times, and found that this phenomenon was unique to mRA vaccination and is different from viral infection.

Plasma Olink Inflammation Panel testing found that IFNγ concentrations increased after the second immunization (Day 21) to 11. 3 times

Clustering of single-cell RNA sequencing data showed that the related bone marrow cell population increased significantly after secondary immunization